KidneyCell

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bioKidneyCell is a startup within the Leonhardt Ventures Cal-X Stars Business Accelerator and Leonhardt’s Launchpads Utah focused on kidney regeneration.

The company utilizes bioelectric stimulation to control release of specific proteins on demand for specific regenerative purposes in sequence:

1. SDF-1 (stem cell homing factor – recruits a persons own stem cells to stimulated tissues).

2. VEGF (for new blood vessel growth).

3  IGF-1 (for DNA repair at the nucleus level). 

4. Follistatin (for muscle and tissue regeneration).

5. RANKL (for demineralization and tissue loosening when needed).

6. Hepatocyte Growth Factor  (tissue regeneration)

7. eNOS (for dilating blood vessels for increasing flow).

8.  Tropoelastin (increases elasticity of any tissues such as skin, arteries, aorta, heart and promotes healing of wounds). 

9. Activin A + B

10.  EGF Epidermal Growth Factor (regeneration)

In extreme cases we combine our regeneration microstimulator with an implantable, programmable, re-fillable stem cell/growth factor micro pump.

In cases of kidney cancer we have special signals to stop tumor cell division and blood supply followed by the above regeneration protocol. 

KidneyCell 

Leonhardt Ventures’ Cal-X Stars Business Accelerator, Inc.

1531 6th Street, Unit 401

Santa Monica, CA  90401

Tel:  310 310 2534

Leonhardt’s Launchpads Utah

489 E, 400 South, Unit 116

Salt Lake City, UT  84111

email:  hleonhardt@aol.com

Contact:  Howard J. Leonhardt 

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Trophic Factors from Tissue Stem Cells for Renal Regeneration

www.hindawi.com/journals/sci/aa/537204/

by K Tsuji – ‎Cited by 3 – ‎Related articles

Jan 9, 2015 – However, regarding renal regeneration, stem cells are discussed to … paracrine including interleukin-6 (IL-6), VEGF, SDF1, and IGF-1 [24].

Role of Growth Factors in Regulation of Renal Growth – Annual Reviews

www.annualreviews.org/doi/pdf/10…/annurev.ph.55.030193.001513

Annual Reviews

by MR Hammerman – ‎1993 – ‎Cited by 43 – ‎Related articles

KEY WORDS: epidermal growth factor, fibroblast growth factor, hepatocyte growth … processes ofkidney growth, regulation of renal function, repair following.

SDF-1

Role of SDF-1 as a regulatory chemokine in renal regeneration after …

www.sciencedirect.com/science/article/pii/S2157171615310194

ScienceDirect

by FE Togel – ‎2011 – ‎Cited by 9 – ‎Related articles

Dec 16, 2015 – Both the homing of hematopoietic stem cells (HSCs) to the bone marrow and their engraftment in recipients of bone marrow transplants are …

Upregulation of Stromal Cell–Derived Factor 1 (SDF-1) is Associated …

www.ncbi.nlm.nih.gov/…/PMC42577…

National Center for Biotechnology Information

by X Wan – ‎2014 – ‎Cited by 3 – ‎Related articles

Dec 5, 2014 – Kidney repair is comprised of surviving epithelial cells that adapt to the loss … In a model of I/R injury induced renal neovascularization, SDF1 …

SDF-1 activates papillary label-retaining cells during kidney repair …

www.jove.com/…/sdf1-activates-papillary-label-ret…

Journal of Visualized Experiments

Jun 7, 2014 – The data indicate that the SDF1/Cxcr4 axis is a critical regulator of papillary LRC activation following transient kidney injury and during organ …

IGF-1

Search Results

`The growth hormone–insulin-like growth factor axis in kidney re …

ndt.oxfordjournals.org/content/14/8/1853.long

by MR Hammerman – ‎1999 – ‎Cited by 34 – ‎Related articles

Recovery is dependent on the ability of the tubular cells to regenerate and … If IGF I were protective for the kidneyone might expect that its administration to rats …

Insulin-like growth factor-1 enhances epidermal growth factor receptor …

www.ncbi.nlm.nih.gov/pubmed/7539476 National Center for Biotechnology Information

by JJ Lin – ‎1995 – ‎Cited by 45 – ‎Related articles

… factor receptor activation and renal tubular cell regeneration in postischemic … Growth factors such as insulin-like growth factor-1 (IGF1), epidermal growth …

Insulin-like growth factors and kidney disease. – NCBI

www.ncbi.nlm.nih.gov/…/25151409

National Center for Biotechnology Information

by LA Bach – ‎2015 – ‎Cited by 15 – ‎Related articles

Aug 21, 2014 – Insulin-like growth factors (IGF1 and IGF-2) are necessary for … IGFs have a potential role in enhancing stem cell repair of kidney injury.

Effects of growth hormone and insulin-like growth factor I on renal …

www.ncbi.nlm.nih.gov/…/9255221

National Center for Biotechnology Information

by MR Hammerman – ‎1997 – ‎Cited by 19 – ‎Related articles

(1)Department of Internal Medicine, Washington University School of Medicine, St. Louis, … growth, and function and participate in the repair processes after renal injury. … Insulin-like growth factor I (IGF-I) accelerates the restoration of kidney …

Enhanced renoprotective effect of IGF-1 modified human umbilical …

www.nature.com › Scientific Reports › Articles Nature

by P Liu – ‎2016 – ‎Cited by 1

Feb 2, 2016 – Our results indicated that the IGF1 overexpression could enhance the … and anti-apoptosis capacity to repair kidney tissues in the rat model of …

Insulin-Like Growth Factor-1 Sustains Stem Cell–Mediated Renal Repair

jasn.asnjournals.org/content/18/11/2921.full

by B Imberti – ‎2007 – ‎Cited by 224 – ‎Related articles

These findings indicate that MSC exert beneficial effects on tubular cell repair in acute kidney injury by producing the mitogenic and pro-survival factor IGF1.

Can hgh regenerate kidney cells? – Steroid.com Forums

forums.steroid.com › … › IGF-1 LR3, HGH, and INSULIN QUESTIONS

Jan 20, 2009 – … IGF1 LR3, HGH, and INSULIN QUESTIONS; Can hgh regenerate … He said I either needed to start dialysis or have a kidney transplant.

EGF – Epidermal Growth Factor

Epidermal growth factor enhances renal tubule cell regeneration and …

www.ncbi.nlm.nih.gov/…/PMC30405…

National Center for Biotechnology Information

by HD Humes – ‎1989 – ‎Cited by 344 – ‎Related articles

Epidermal growth factor enhances renal tubule cell regeneration and repair and accelerates the recovery of renal function in postischemic acute renal failure.

Hepatocyte Growth Factor

Hepatocyte growth factor in renal regeneration, renal disease and …

www.ncbi.nlm.nih.gov/…/10926176

National Center for Biotechnology Information

by K Matsumoto – ‎2000 – ‎Cited by 64 – ‎Related articles

Curr Opin Nephrol Hypertens. 2000 Jul;9(4):395-402. Hepatocyte growth factor in renal regenerationrenal disease and potential therapeutics. Matsumoto K(1) …

Hepatocyte growth factor in renal failure: promise and reality. – NCBI

www.ncbi.nlm.nih.gov/…/10760078

National Center for Biotechnology Information

by GA Vargas – ‎2000 – ‎Cited by 118 – ‎Related articles

In the case of Prometheus, we can now suppose that his amazing hepatic regeneration was caused by a peptide growth factor called hepatocyte growth factor …

Hepatocyte growth factor: renotropic role and potential therapeutics for …

www.ncbi.nlm.nih.gov/…/11380804

National Center for Biotechnology Information

by K Matsumoto – ‎2001 – ‎Cited by 377 – ‎Related articles

Hepatocyte growth factor: renotropic role and potential therapeutics for renal diseases. … Locally and systemically increased HGF supports renal regeneration, …

Hepatocyte growth factor in kidney fibrosis: therapeutic potential and …

www.ncbi.nlm.nih.gov/…/15180923

National Center for Biotechnology Information

by Y Liu – ‎2004 – ‎Cited by 267 – ‎Related articles

Hepatocyte growth factor (HGF) is a pleiotropic factor that plays an imperative role in tubular repairand regeneration after acute renal injury. Growing evidence ..

Activin

Activin-like kinase 3 is important for kidney regeneration and … – NCBI

www.ncbi.nlm.nih.gov/…/22306733

National Center for Biotechnology Information

by H Sugimoto – ‎2012 – ‎Cited by 89 – ‎Related articles

Nat Med. 2012 Feb 5;18(3):396-404. doi: 10.1038/nm.2629. Activin-like kinase 3 is important forkidney regeneration and reversal of fibrosis. Sugimoto H(1) .

Follistatin

Involvement of the activin-follistatin system in tubular regeneration …

www.ncbi.nlm.nih.gov/…/11461941

National Center for Biotechnology Information

by A Maeshima – ‎2001 – ‎Cited by 63 – ‎Related articles

This study was conducted to investigate the involvement of the activin-follistatin system in renal regeneration after ischemic injury. Expression of mRNA for the …

The role of the activin-follistatin system in the developmental … – NCBI

www.ncbi.nlm.nih.gov/…/11544099 National Center for Biotechnology Information

by A Maeshima – ‎2001 – ‎Cited by 32 – ‎Related articles

Cytokine Growth Factor Rev. 2001 Dec;12(4):289-98. The role of the activin-follistatin system in the developmental and regeneration processes of the kidney.

Stem Cells

Stem cells in kidney regeneration. – NCBI

www.ncbi.nlm.nih.gov/…/22963572

National Center for Biotechnology Information

by S Yokote – ‎2012 – ‎Cited by 5 – ‎Related articles

Curr Med Chem. 2012;19(35):6009-17. Stem cells in kidney regeneration. Yokote S(1), Yokoo T. Author information: (1)Division of Nephrology and Hypertension …

Scientists Report Breakthrough in Growing Kidneys from Stem Cells

www.healthline.com › Healthline News

Healthline Networks

Sep 23, 2015 – Scientists Report Breakthrough in Growing Kidneys from Stem Cells … “Several groups, including us, have reported regeneration of kidney, …

Regrow or Repair: Potential Regenerative Therapies for the Kidney

jasn.asnjournals.org/content/17/9/2390.full

by MH Little – ‎2006 – ‎Cited by 129 – ‎Related articles

Other in situ possibilities include the recruitment of stem cells to the kidney to elicit repair and the induction of dedifferentiation of resident renal cells. Whereas …

Electrical Stimulation

Acupuncture with electrical stimulation may treat … – Medical Xpress

medicalxpress.com › Diseases, Conditions, Syndromes

Aug 14, 2014 – Acupuncture with electrical stimulation may treat muscle atrophy caused … result ofkidney and other diseases, according to a study appearing in an … The researchers found that the treatment improved muscle regeneration in …

Acupuncture plus Low-Frequency Electrical Stimulation (Acu-LFES …

journals.plos.org/plosone/article?id=10.1371/journal.pone.0134511

by Z Su – ‎2015 – ‎Cited by 4 – ‎Related articles

Jul 31, 2015 – Muscle regeneration capacity was significantly increased by Acu-LFES. … (2015) Acupuncture plus Low-Frequency Electrical Stimulation …… Exercise ameliorates chronic kidneydisease-induced defects in muscle protein ..

 

Adult kidneys constantly grow, remodel themselves, study finds | News …

Jun 19, 2014 – “These are basic findings that have direct implications for kidney disease and kidney regeneration,” said Yuval Rinkevich, PhD, the lead author …

 

Researchers make step towards kidney regeneration by developing …

Jun 7, 2016 – Researchers have established methods for culturing kidney progenitor cells in vitro that maintain their ability to form the glomerulus.

 

Need A New Kidney? Let’s Regenerate One! – Asian Scientist Magazine

www.asianscientist.com/…/in…/kidney-progenitor-cell-regeneration-tissue-engineerin…

Jun 15, 2016 – Under the correct conditions, kidney progenitor cells can be induced to produce more glomeruli and tubules, the building blocks of the kidney.

Expansion of kidney progenitor cells toward regenerative medicine …

Jun 6, 2016 – The kidney is a difficult organ to regenerate. However, in a big step forward for kidney regeneration research, a collaboration between scientists …

 
Human iPS-Derived Kidneys Successfully Hook Up To Blood Vessels In a world first, researchers have successfully connected mouse kidney capillaries with kidney tissue derived from human induced pluripotent stem cells. Human iPSC Used To Treat Kidney Injury In Mice A public-private partnership between CiRA and Astellas has yielded the finding that iPSC could be used to treat acute kidney injury. Read more from Asian Scientist Magazine at: http://www.asianscientist.com/2016/06/in-the-lab/kidney-progenitor-cell-regeneration-tissue-engineering/
 2012 Jul 20;21(11):1911-23. doi: 10.1089/scd.2011.0333. Epub 2011 Dec 23.

Human amniotic fluid stem cell preconditioning improves their regenerative potential.

Abstract

Human amniotic fluid stem (hAFS) cells, a novel class of broadly multipotent stem cells that share characteristics of both embryonic and adult stem cells, have been regarded as promising candidate for cell therapy. Taking advantage by the well-established murine model of acute kidney injury (AKI), we studied the proregenerative effect of hAFS cells in immunodeficient mice injected with the nephrotoxic drug cisplatin. Infusion of hAFS cells in cisplatin mice improved renal function and limited tubular damage, although not to control level, and prolonged animal survival. Human AFS cells engrafted injured kidney predominantly in peritubular region without acquiring tubular epithelial markers. Human AFS cells exerted antiapoptotic effect, activated Akt, and stimulated proliferation of tubular cells possibly via local release of factors, including interleukin-6, vascular endothelial growth factor, and stromal cell-derived factor-1, which we documented in vitro to be produced by hAFS cells. The therapeutic potential of hAFS cells was enhanced by cell pretreatment with glial cell line-derived neurotrophic factor (GDNF), which markedly ameliorated renal function and tubular injury by increasing stem cell homing to the tubulointerstitial compartment. By in vitro studies, GDNF increased hAFS cell production of growth factors, motility, and expression of receptors involved in cell homing and survival. These findings indicate that hAFS cells can promote functional recovery and contribute to renal regeneration in AKI mice via local production of mitogenic and prosurvival factors. The effects of hAFS cells can be remarkably enhanced by GDNF preconditioning.
PMID:
22066606
PMCID:
PMC3396139
DOI:
10.1089/scd.2011.0333
[Indexed for MEDLINE]

Free PMC Article

 2010 Oct;177(4):2011-21. doi: 10.2353/ajpath.2010.091245. Epub 2010 Aug 19.

Stem cells derived from human amniotic fluid contribute to acute kidney injury recovery.

Abstract

Stem cells isolated from human amniotic fluid are gaining attention with regard to their therapeutic potential. In this work, we investigated whether these cells contribute to tubular regeneration after experimental acute kidney injury. Cells expressing stem cell markers with multidifferentiative potential were isolated from human amniotic fluid. The regenerative potential of human amniotic fluid stem cells was compared with that of bone marrow-derived human mesenchymal stem cells. We found that the intravenous injection of 3.5 × 10(5) human amniotic fluid stem cells into nonimmune-competent mice with glycerol-induced acute kidney injury was followed by rapid normalization of renal function compared with injection of mesenchymal stem cells. Both stem cell types showed enhanced tubular cell proliferation and reduced apoptosis. Mesenchymal stem cells were more efficient in inducing proliferation than amniotic fluid-derived stem cells, which, in contrast, were more antiapoptotic. Both cell types were found to accumulate within the peritubular capillaries and the interstitium, but amniotic fluid stem cells were more persistent than mesenchymal stem cells. In vitro experiments demonstrated that the two cell types produced different cytokines and growth factors, suggesting that a combination of different mediators is involved in their biological actions. These results suggest that the amniotic fluid-derived stem cells may improve renal regeneration in acute kidney injury, but they are not more effective than mesenchymal stem cells.
PMID:
20724594
PMCID:
PMC2947295
DOI:
10.2353/ajpath.2010.091245
[Indexed for MEDLINE]

Free PMC Articl

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